abstract：:Langerhans cells appear to be critical for IgE-mediated allergen capture and presentation in human atopic dermatitis. The present study sought to determine whether epidermal (i.e Langerhans cells) and dermal dendritic cells in the skin of dogs with atopic dermatitis are hyperplastic and expressed surface IgE. Frozen s...

journal_title：Archives of dermatological research

authors： Olivry T,Moore PF,Affolter VK,Naydan DK

更新日期：1996-09-01 00:00:00

abstract：:Interferon-alpha combined with retinoid or PUVA is used for the treatment of cutaneous T-cell lymphoma. Anti-IFN-alpha antibodies (IFN ab) occur regularly during IFN-alpha treatment. We investigated the incidence of neutralizing and binding IFN ab and analysed their relationship with clinical and immunological paramet...

journal_title：Archives of dermatological research

authors： Rajan GP,Seifert B,Prümmer O,Joller-Jemelka HI,Burg G,Dummer R

更新日期：1996-08-01 00:00:00

abstract：:Interleukin-6-like (IL-6-like) immunoreactivity was sought in inflamed and normal human skin using the same immunohistochemical technique as for detection of neuropeptides. Such immunoreactivity was found in dermal and in a few intraepidermal nerve-like fibres in biopsy specimens from inflamed skin from patients with ...

journal_title：Archives of dermatological research

authors： Nordlind K,Chin LB,Ahmed AA,Brakenhoff J,Theodorsson E,Lidén S

更新日期：1996-07-01 00:00:00

abstract：:A low molecular mass cysteine proteinase inhibitor was found and isolated from bovine hoof. Purification of the inhibitor to homogeneity was performed by carboxymethyl-papain-sepharose affinity and DE-52 ion exchange chromatographies. From 15 g of bovine hoof, 0.25 mg of cysteine proteinase inhibitor was isolated. It ...

journal_title：Archives of dermatological research

authors： Tsushima H,Higashiyama K,Mine H

更新日期：1996-07-01 00:00:00

abstract：:Pemphigus vulgaris (PV) and pemphigus foliaceus (PF) are autoimmune blistering diseases characterized by a loss of cell-cell adhesion and by autoantibodies directed against epidermal cadherins. PF antigen has been established as desmoglein I which is located strictly on the desmosome, whereas the precise ultrastructur...

journal_title：Archives of dermatological research

authors： Bédane C,Prost C,Thomine E,Intrator L,Joly P,Caux F,Blecker M,Bernard P,Leboutet MJ,Tron F,Lauret P,Bonnetblanc JM,Dubertret L

更新日期：1996-06-01 00:00:00

abstract：:The fetal development of Merkel cells-neuroendocrine cells of the skin - has been a matter of debate for a long time. Recent results have helped to confirm their intraepidermal development in humans. Simple epithelial cytokeratins (CK) nos. 8, 18, 19 and 20 are well established markers at the light microscopic level. ...

journal_title：Archives of dermatological research

authors： Moll I,Zieger W,Schmelz M

更新日期：1996-04-01 00:00:00

abstract：:Certain HPV types have been linked to the genesis and development of premalignant and malignant skin diseases. There have been several contradictory reports on the role of HPV infections in the development of keratoacanthomas (KAs). To further study the involvement of HPVs in the aetiology of KAs, we investigated para...

journal_title：Archives of dermatological research

authors： Lu S,Syrjänen SL,Havu VK,Syrjänen S

更新日期：1996-03-01 00:00:00

abstract：:Using immunoblot and immunofluorescence analysis, we demonstrated the presence and localization of an immunoreactive form of erythrocyte ankyrin in human epidermal keratinocytes. Immunoblot analysis revealed that both human epidermis and cultured epidermal keratinocytes contained ankyrin-like proteins of molecular mas...

journal_title：Archives of dermatological research

authors： Shimizu T,Takakuwa Y,Koizumi H,Ishibashi T,Ohkawara A

更新日期：1996-01-01 00:00:00

abstract：:Costimulation of anti-CD3-triggered proliferative T-cell responses by type I and type IV collagen and fibronectin was studied in 25 patients with psoriasis and 12 healthy subjects. The stimulation index of anti-CD3-mediated responses in the presence of type I collagen was about half that in the controls. Although the ...

journal_title：Archives of dermatological research

authors： Glinski W,Gorski A,Glinska-Ferenz M,Majewski S,Stepien-Sopniewska B

更新日期：1995-01-01 00:00:00

abstract：:In this study we investigated the influence of keratinocytes on the phenotype of fibroblasts in an in vitro human skin equivalent. Keratinocytes were seeded at the surface of fibroblast-populated mechanically restrained type I collagen gels (lattices). Lattices without keratinocytes were handled in parallel as control...

journal_title：Archives of dermatological research

authors： Lacroix M,Bovy T,Nusgens BV,Lapière CM

更新日期：1995-01-01 00:00:00

abstract：:Secretory component (SC) acts as a transmembrane polymeric immunoglobulin receptor of epithelial cells and is known to bind to polymeric IgA and to contribute to the secretion of secretory IgA (sIgA). We describe a new biological function for free SC (FSC) by which the expression of intercellular adhesion molecule-1 (...

journal_title：Archives of dermatological research

authors： Nihei Y,Maruyama K,Zhang JZ,Kobayashi K,Kaneko F

更新日期：1995-01-01 00:00:00

abstract：:Epstein-Barr virus (EBV) can infect epithelial cells as well as B lymphocytes. Infection of the male and female genital tracts has recently been demonstrated, and it has been suggested that the virus may be sexually transmissible. In our study we investigated whether EBV can be found in the anal region of sexually act...

journal_title：Archives of dermatological research

authors： Näher H,Lenhard B,Wilms J,Nickel P

更新日期：1995-01-01 00:00:00

abstract：:Epidermolytic hyperkeratosis (EH) is a genetic disorder of keratins associated with epidermal differentiation. Affected individuals carry gene mutations for conserved sequences of keratins K1 or K10. The structural alterations of tonofilaments in EH seem to be a direct consequence of the keratin gene mutations. EH epi...

journal_title：Archives of dermatological research

authors： Ishida-Yamamoto A,Iizuka H,Manabe M,O'Guin WM,Hohl D,Kartasova T,Kuroki T,Roop DR,Eady RA

更新日期：1995-01-01 00:00:00

abstract：:In order to improve the characterization of human malignant melanoma cells and their variant gene expression in vitro, a search for specifically regulated genes was performed. Four melanoma cell lines (M5, MEWO, IGR39, SKMEL13) and newly cultured normal human melanocytes were included in a comparative hybridization (d...

journal_title：Archives of dermatological research

authors： Eberle J,Garbe C,Orfanos CE

更新日期：1995-01-01 00:00:00

abstract：:The rhino mouse comedolytic model and the hairless mouse photoaging model are established animal models for screening the in vivo activity of retinoids. However, the expression of the retinoic acid receptors (RARs) and cellular retinoic acid-binding proteins (CRABPs), known to regulate retinoid activity, is not comple...

journal_title：Archives of dermatological research

authors： Beehler BC,Chen S,Tramposch KM

更新日期：1995-01-01 00:00:00

abstract：:Six patients with delayed pressure urticaria (DPU) applied clobetasol propionate (0.05%) ointment or its base to predetermined test sites on the right and left thigh as part of a randomized, double-blind study. A pressure challenge was administered to each test site at the initial visit and repeated after 3 days and 6...

journal_title：Archives of dermatological research

authors： Barlow RJ,Macdonald DM,Black AK,Greaves MW

更新日期：1995-01-01 00:00:00

abstract：:In the present study the action of various cytokines as regulators of human melanocyte growth and differentiation was examined in vitro. Primary melanocyte cultures were obtained in complete medium free of 12-O-tetradecanoylphorbol-13-acetate or serum. First passage melanocytes were treated with various concentrations...

journal_title：Archives of dermatological research

authors： Krasagakis K,Garbe C,Eberle J,Orfanos CE

更新日期：1995-01-01 00:00:00

abstract：:Hydroperoxides of d-limonene were shown to be potent contact allergens when studied in guinea-pigs. Limonene-2-hydroperoxide (2-hydroperoxy-p-mentha-6,8-diene, a mixture of trans and cis isomers) was synthesized for the first time. The ratio between the trans and cis forms was 3:1. These two hydroperoxides were identi...

journal_title：Archives of dermatological research

authors： Karlberg AT,Shao LP,Nilsson U,Gäfvert E,Nilsson JL

更新日期：1994-01-01 00:00:00

abstract：:The incidence of cutaneous malignant melanoma continues to rise. Strategies to reduce mortality in this situation include improving therapy, encouraging earlier diagnosis, and promoting prevention. New developments such as epiluminescence, sentinel node biopsy, arterial limb perfusion and the use of vaccines are discu...

journal_title：Archives of dermatological research

authors： MacKie RM

更新日期：1994-01-01 00:00:00

abstract：:The demonstration of cytokine mRNA expression in epidermal cells by the polymerase chain reaction technique preceded by reverse transcription (RT-PCR) requires linear test conditions (i.e. that the product obtained after amplification reflects the relative amounts of starting material) and high reproducibility, specif...

journal_title：Archives of dermatological research

authors： Enk CD,Katz SI

更新日期：1994-01-01 00:00:00

abstract：:Fast antigen is a cell surface protein that mediates apoptosis. Using immunohistological, flow cytometry and electron microscopic analyses, we investigated the expression of Fas antigen on various skin tissues, and on cultured SV40-transformed human epidermal keratinocyte cell line KJD and human skin squamous cell car...

journal_title：Archives of dermatological research

authors： Oishi M,Maeda K,Sugiyama S

更新日期：1994-01-01 00:00:00

abstract：:The treatment of epidermolysis bullosa acquisita (EBA) is difficult and often disappointing. We report on the successful treatment of two EBA patients with colchicine. The drug was administered orally at an initial dose of 2 mg/day. After 2 weeks of therapy a dramatic improvement was observed. Most of the cutaneous an...

journal_title：Archives of dermatological research

authors： Megahed M,Scharffetter-Kochanek K

更新日期：1994-01-01 00:00:00

abstract：:The effects of acidic and basic fibroblast growth factors (aFGF and bFGF, respectively) on proliferation and differentiation of cultured hair apparatus cells were examined using bovine pituitary extract (BPE)-free media. The importance of FGFs as growth factors in BPE was also examined. Both FGFs and BPE stimulated ce...

journal_title：Archives of dermatological research

authors： Tanigaki-Obana N,Ito M

更新日期：1994-01-01 00:00:00

abstract：:Adult female Wistar rats (n = 48) divided into four groups of 12 were treated orally with 3 mg/kg per day thalidomide, a 0.05% hexachlorobenzene (HCB)--containing diet, with both drugs together and with the vehicles (controls) over periods of 10 and 60 days. The protein and P450 contents and the activities of aminopyr...

journal_title：Archives of dermatological research

authors： Tsambaos D,Bolsen K,Georgiou S,Monastirli A,Goerz G

更新日期：1994-01-01 00:00:00

abstract：:Mitochondrial DNA (mtDNA) mutations have recently been proposed as important contributors to ageing and degenerative diseases. In this study, we applied the polymerase chain reaction technique to detect 4.977 bp-deleted mtDNA, the most common and abundant deletion of mtDNA, in normal human skin. None of the skin speci...

journal_title：Archives of dermatological research

authors： Yang JH,Lee HC,Lin KJ,Wei YH

更新日期：1994-01-01 00:00:00

abstract：:Nickel, cobalt and chromium are metals very often implicated in allergic contact dermatitis. In vivo, keratinocytes, which are the first target cells, can be directly activated to participate in the local reaction, especially through the expression of the membrane antigen ICAM-1, a ligand of the leucocyte antigen LFA-...

journal_title：Archives of dermatological research

authors： Guéniche A,Viac J,Lizard G,Charveron M,Schmitt D

更新日期：1994-01-01 00:00:00

abstract：:The immigration of circulating T cells into specific tissues is directed by the interaction between adhesion molecules on lymphocyte subpopulations and their ligands on vascular endothelium. Of these, endothelial leucocyte adhesion molecule (ELAM-1), weakly expressed in normal human skin (NHS), seems to be the counter...

journal_title：Archives of dermatological research

authors： Bos JD,de Boer OJ,Tibosch E,Das PK,Pals ST

更新日期：1993-01-01 00:00:00

abstract：:The cellular DNA ploidy of 30 cases of malignant melanoma was measured by 4',6-diamidino-2-phenylindole-DNA (DAPI-DNA) microfluorometry. DNA histograms and DNA index values were compared among melanomas of different cell morphology. Epithelioid cell melanomas often showed greater DNA aneuploidy than spindle cell melan...

journal_title：Archives of dermatological research

authors： Chi HI,Uyeda Y,Umebayashi Y,Otsuka F

更新日期：1993-01-01 00:00:00

abstract：:Filaggrin, a basic protein of the stratum corneum, was named as such because of its capability to aggregate keratin intermediate filaments in vitro. To investigate its filament-aggregating capability in vivo, we performed immunoelectron microscopy in three autosomal dominant genodermatoses serving as in vivo models of...

journal_title：Archives of dermatological research

authors： Weidenthaler B,Hausser I,Anton-Lamprecht I

更新日期：1993-01-01 00:00:00

abstract：:Recent studies have demonstrated that tumor necrosis factor-alpha (TNF-alpha) selectively decreases production of collagens I and III, the major types of collagen in the dermis, and increases production of collagenase in cultured dermal fibroblasts. The effects of TNF-alpha on collagens I, III and VI, fibronectin and ...

journal_title：Archives of dermatological research

authors： Takeda K,Hatamochi A,Arakawa M,Ueki H

更新日期：1993-01-01 00:00:00

abstract：:The deposition of immunoglobulin (Ig) at the dermo-epidermal junction (DEJ) of the skin, frequently observed in autoimmune mouse strains, is similar to that seen in patients with systemic lupus erythematosus (SLE). MRL/Mp-lpr/lpr (MRL/lpr) mice have an autosomal recessive mutant gene, lpr, which produces massive T-cel...

journal_title：Archives of dermatological research

authors： Furukawa F,Ohshio G,Imamura S

更新日期：1993-01-01 00:00:00

abstract：:Keratin specificity analyses of eight anti-keratin antibodies (34 beta B4 (K1), 35 beta H11 (K8), Ks 13.1 (K13), Ks 19.1 (K19), PKK1, LP34 (CK1), KL1 and AE1) using keratin protein derived from normal thigh epidermis, normal parotid gland and a human squamous cell carcinoma cell line (HSC-5) were performed, and compar...

journal_title：Archives of dermatological research

authors： Ansai SI,Katagata Y,Yoshikawa KI,Hozumi Y,Aso K

更新日期：1993-01-01 00:00:00

abstract：:The expression and location of the Ki-67 antigen was investigated in 62 basal cell carcinomas (BCC) using immunostaining techniques (PAP and APAAP) on cryostat sections. The tumor samples were classified into three groups according to microarchitecture (nodular, superficial or fibrosing). The Ki-67 growth fraction dis...

journal_title：Archives of dermatological research

authors： Baum HP,Meurer I,Unteregger G

更新日期：1993-01-01 00:00:00

abstract：:The presence, distribution and cellular localization of tumour necrosis factor-alpha (TNF alpha) were investigated in normal human and murine epidermis using immunohistological and immunoelectron microscopic methods with monoclonal and polyclonal antibodies. The immunostaining revealed an intercellular plasma membrane...

journal_title：Archives of dermatological research

authors： Kolde G,Schulze-Osthoff K,Meyer H,Knop J

更新日期：1992-01-01 00:00:00

abstract：:To examine the induction and repair of UV-induced DNA damage, indirect immunofluorescence was performed on UVB-irradiated organ-cultured normal human skin using monoclonal antibodies specific for either cyclobutane pyrimidine dimers or (6-4) photoproducts. Nuclear immunofluorescence of cyclobutane pyrimidine dimers an...

journal_title：Archives of dermatological research

authors： Muramatsu T,Kobayashi N,Tada H,Yamaji M,Shirai T,Mori T,Ohnishi T

更新日期：1992-01-01 00:00:00

abstract：:For the better part of the past century, dermatologists have regarded the skin primarily as a large protective coat. Epidermal keratinocytes were highlighted for their production of keratins and lipids, which contributed to the structural integrity and barrier formation of skin. This "saran-wrap" perspective of skin m...

journal_title：Archives of dermatological research

authors： Nickoloff BJ

更新日期：1992-01-01 00:00:00

abstract：:Interleukin-6 (IL-6) is a multifunctional cytokine which has been suggested to function as an autocrine mitogen in psoriatic epidermis. We report here the results of several experiments designed to further examine this hypothesis. Blot hybridization was unable to detect 1.3 kb IL-6 transcripts in RNA extracted from no...

journal_title：Archives of dermatological research

authors： Elder JT,Sartor CI,Boman DK,Benrazavi S,Fisher GJ,Pittelkow MR

更新日期：1992-01-01 00:00:00

abstract：:Bradykinin has long been postulated to have a major role in physiologic human pain production. We have developed a method to systematically quantify the algogenic response to bradykinin in human skin by the direct application of bradykinin to suction blister bases. After determination of the pain threshold to bradykin...

journal_title：Archives of dermatological research

authors： Schneider BK,Morelli JG,Vavrek RJ,Stewart JM,Weston WL

更新日期：1992-01-01 00:00:00

abstract：:Koebner response was studied in 35 psoriatic patients. Two punch biopsies per patient were taken from non-lesional psoriatic skin before, and 6 h, 2 days, 7 days, 14 days and 21 days after, tape stripping. Alterations in the numbers of CD1+ Langerhans cells, CD4+ and CD8+ T cells and CD11c+ macrophages were mapped mor...

journal_title：Archives of dermatological research

authors： Paukkonen K,Naukkarinen A,Horsmanheimo M

更新日期：1992-01-01 00:00:00

abstract：:Centromere protein B (CENP-B), which is an alphoid DNA binding protein, is the target antigen in autoimmune disease patients (often those with scleroderma). In this study, we analysed activities of anti-CENP-B-DNA complex in anticentromere antibody (ACA)-positive patients using DNA immunoprecipitation with purified CE...

journal_title：Archives of dermatological research

authors： Muro Y,Matsumoto Y,Ohashi M

更新日期：1992-01-01 00:00:00